To gain a better understanding of Brettanomyces yeast during semi-aerobic conditions, studies were conducted observing daily growth during batch culture propagation. The main objective of the study was to create an ideal standard technique which could be used for propagating Brettanomyces spp. in large enough quantities which were physically ready to begin fermentation. Prior to the study no information existed on the specific propagation of Brettanomyces yeast for use in fermentation. By modeling growth during batch culture a propagation method slightly unique to Brettanomyces spp. was developed and effectively used for obtaining yeast cells necessary throughout the rest of the research. The following links detail the methods used to conduct the studies and their subsequent results with discussion.
Methods used to study growth during batch culture propagation.
Results obtained from studying growth during batch culture propagation.
Discussion of the results obtained and their importance in Brettanomyces spp. propagation.